hepg2 human hepatocellular carcinoma cells  (ATCC)

Journal: Chemmedchem

Article Title: Structure‐Based Discovery of Obeticholic Acid Derivatives as Novel Farnesoid X Receptor Partial Agonists with Improved Selectivity and Reduced Off‐Target Effects

doi: 10.1002/cmdc.202500960

Figure Lengend Snippet: Results of RT‐PCR in HepG2 cell line following overnight treatment with endogenous FXR agonist CDCA ( red ) (30 μM), OCA ( purple ) (10 µM), compound 2 ( green ) (10 μM and 30 μM), or compound 16 ( blue ) (10 μM and 30 μM). Not treated cells ( white ) were stimulated with DMSO 0.1. % Statistical significance was analyzed by One‐way Anova‐GraphPad Prism 5. ns = not significant, *p < 0.05, **p < 0.01, ***p < 0.001 vs 0.1% NT = not treated (DMSO).

Article Snippet: HepG2 human hepatocellular carcinoma cells (RRID:CVCL_0027, HB‐8065, ATCC) were cultured in E‐MEM medium supplemented with 1% penicillin/streptomycin, 1% L‐glutamine, and 10% fetal bovine serum (Life Technologies).

Techniques: Reverse Transcription Polymerase Chain Reaction

Journal: Biomaterials

Article Title: A novel Kupffer cell-targeting nanoparticle system to Mitigate alcohol-associated liver disease

doi: 10.1016/j.biomaterials.2025.123623

Figure Lengend Snippet: Release of DEX from NPs in 1X PBS for 21 days at 37°C and two different pH: 6.0 and 7.4; (a) PC-0.2+DEX; (b) PC-0.5+DEX; (c) PC-1+DEX: only PC-1+DEX showed significantly high pH-dependent release of DEX; n = 3 (d) cytocompatibility studies in (i) HepG2 cells and (ii) DTHP1 (macrophages) confirmed cytocompatibility of PC-1 and PC-1+INT NPs in both cell lines 24 h after treatment; n = 4.

Article Snippet: Human hepatocellular carcinoma cell line HepG2 cells were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques:

Journal: Biomaterials

Article Title: A novel Kupffer cell-targeting nanoparticle system to Mitigate alcohol-associated liver disease

doi: 10.1016/j.biomaterials.2025.123623

Figure Lengend Snippet: Uptake of NPs by DTHP1 and HepG2 cells treated with 1 μg/ml LPS and 0.16 % (v/v) EtOH; (a) quantitative analysis of NP uptake at different concentrations, confirmed higher, dose-dependent uptake by DTHP1 cells.; n = 4 (b) fluorescence imaging of NP uptake also confirmed higher uptake in DTHP1 than in HepG2 cells: green-coumarin-6 loaded NPs, blue-nuclei (scale bar = 150 μm) (c) quantification of NP uptake after cells were pre-treated with free INT (0.1 μM and 1 μM); uptake of the PC-1+INT NPs reduced due to the free INT pretreatment, confirming Gpbar1 targeting capabilities of the NPs.

Article Snippet: Human hepatocellular carcinoma cell line HepG2 cells were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Fluorescence, Imaging